{"id":1555,"date":"2018-08-11T18:06:55","date_gmt":"2018-08-11T09:06:55","guid":{"rendered":"http:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/?page_id=1555"},"modified":"2018-10-03T12:36:51","modified_gmt":"2018-10-03T03:36:51","slug":"alife","status":"publish","type":"page","link":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/topics\/alife\/","title":{"rendered":"\u4eba\u5de5\u751f\u547d\u3092\u3064\u304f\u308b \u301c\u5408\u6210\u751f\u7269\u5b66\u301c"},"content":{"rendered":"<div id=\"attachment_1724\" style=\"width: 310px\" class=\"wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-1724\" class=\"size-medium wp-image-1724\" src=\"http:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-content\/uploads\/sites\/57\/2018\/10\/tricolor-small-300x268.jpg\" alt=\"\" width=\"300\" height=\"268\" srcset=\"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-content\/uploads\/sites\/57\/2018\/10\/tricolor-small-300x268.jpg 300w, https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-content\/uploads\/sites\/57\/2018\/10\/tricolor-small-660x590.jpg 660w, https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-content\/uploads\/sites\/57\/2018\/10\/tricolor-small.jpg 674w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-1724\" class=\"wp-caption-text\">LACE-PCR\u7523\u7269\u3067\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u3092\u3057\u305f\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u3092\u767a\u73fe\u3059\u308b\u5927\u8178\u83cc<\/p><\/div>\n<p><span style=\"font-size: large\">ATGC<\/span>\u4ee5\u5916\u306e\u5929\u7136\u306b\u5b58\u5728\u3057\u306a\u3044\u300c\u6587\u5b57\u300d\u3092\u4f7f\u3063\u3066\u79c1\u305f\u3061\u304c\u958b\u767a\u3057\u305f\u6280\u8853\u306e\u3072\u3068\u3064\u306b\u3001\u300c<strong>\u7a81\u51fa\u672b\u7aef\u751f\u6210PCR\u6cd5<\/strong>\u300d\u304c\u3042\u308a\u307e\u3059\u3002\u82f1\u8a9e\u3067\u300c<strong>LACE (Ligation Assisted Cohesive Ending) -PCR\u6cd5<\/strong>\u300d\u3068\u540d\u4ed8\u3051\u305f\u3053\u306e\u65b9\u6cd5\u306f\u3001PCR\u3067\u4f7f\u7528\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u5149\u958b\u88c2\u6027\u306e\u4fdd\u8b77\u57fa\u3092\u5c0e\u5165\u3057\u305f\u5869\u57fa\uff08<strong>\u30b1\u30fc\u30b8\u30c9\u6838\u9178<\/strong>\uff09\u3092\u4f7f\u7528\u3057\u307e\u3059\uff08\u7279\u8a31\u7b2c5397960\u53f7\uff09\u3002\u3053\u306e\u7279\u6b8a\u5869\u57fa\u304cDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u308b\u8907\u88fd\u53cd\u5fdc\u3092\u4f4d\u7f6e\u7279\u7570\u7684\u306b\u963b\u5bb3\u3059\u308b\u3053\u3068\u3067\u3001\u3053\u306e\u7279\u6b8a\u5869\u57fa\u3088\u308a\u30825&#8217;\u7aef\u5074\u306b\u3042\u308b\u9818\u57df\u304c\u4fdd\u8b77\u3055\u308c\u3001PCR\u53cd\u5fdc\u3092\u901a\u3058\u3066\u5e38\u306b\uff11\u672c\u9396\u306e\u72b6\u614b\u3092\u4fdd\u3061\u307e\u3059\u3002\u6700\u5f8c\u306b\u7d2b\u5916\u7dda\u3092\u77ed\u6642\u9593\u7167\u5c04\u3057\u3066\u5149\u958b\u88c2\u6027\u4fdd\u8b77\u57fa\u3092\u9664\u53bb\u3059\u308c\u3070\u3001\u306e\u305e\u307f\u306e\u914d\u5217\u3001\u306e\u305e\u307f\u306e\u9577\u3055\u306e\u7a81\u51fa\u672b\u7aef\u3092\u6709\u3059\u308bPCR\u7523\u7269\u304c\u3001\u975e\u5e38\u306b\u7c21\u4fbf\u306b\u5f97\u3089\u308c\u308b\u306e\u3067\u3059\u3002<\/p>\n<p>\u79c1\u305f\u3061\u306f\u3053\u308c\u307e\u3067\u306b\u3001\u5927\u8178\u83cc\u306b\u5bc4\u751f\u3059\u308b\u300c<strong>\u30d7\u30e9\u30b9\u30df\u30c9<\/strong>\u300d\u3068\u3044\u3046\u74b0\u72b6DNA\u306e\u5168\u9577\uff08\u7d045,000\u5869\u57fa\u5bfe\uff09\u3092LACE-PCR\u3067\u5897\u5e45\u3057\u3001\u3053\u308c\u4ee5\u4e0a\u306e\u4e00\u5207\u306e\u9175\u7d20\u53cd\u5fdc\u3092\u5fc5\u8981\u3068\u3057\u306a\u3044\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u6cd5\uff08<strong>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u975e\u4f9d\u5b58\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6cd5\uff08LIC\uff09<\/strong>\uff09\u3078\u3068\u5fdc\u7528\u3057\u307e\u3057\u305f\uff08<a href=\"https:\/\/doi.org\/10.3390\/molecules17010328\" target=\"_blank\" rel=\"noopener\"><em>Molecules<\/em>, <strong>2012<\/strong>, <em>17<\/em>, 328<\/a>\uff09\u3002\u4eca\u5f8c\u306f\u3001\u3084\u306f\u308a\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u3067\u5229\u7528\u3055\u308c\u308bM13\u30d5\u30a1\u30fc\u30b8\u30a6\u30a3\u30eb\u30b9\uff08\u5168\u9577\u7d048,000\u5869\u57fa\u5bfe\uff09\u3084\u03bb\u30d5\u30a1\u30fc\u30b8\uff08\u7d0448,000\u5869\u57fa\u5bfe\uff09\u3092LACE-PCR\u3067\u5897\u5e45\u3059\u308b\u3053\u3068\u3067\u3001\u30a6\u30a3\u30eb\u30b9\u30b2\u30ce\u30e0\u306e\u5b8c\u5168\u4eba\u5de5\u5408\u6210\u3092\u8a66\u307f\u307e\u3059\u3002\u305d\u3057\u3066\u5c06\u6765\u7684\u306b\u306f\u3001\u30de\u30a4\u30b3\u30d7\u30e9\u30ba\u30de\uff08\u7d04580,000\u5869\u57fa\u5bfe\uff09\u3084\u5927\u8178\u83cc\uff08\u7d044,600,000\u5869\u57fa\u5bfe\uff09\u306a\u3069\u306e<strong>\u539f\u6838\u751f\u7269\u306e\u30b2\u30ce\u30e0\u3092\u5b8c\u5168\u5408\u6210<\/strong>\u3059\u308b\u3053\u3068\u3067\u3001\u300c\u4eba\u306e\u624b\u3067\u4e00\u304b\u3089\u3064\u304f\u308a\u3042\u3052\u305f\u751f\u7269\uff1d<strong>\u4eba\u5de5\u751f\u547d<\/strong>\u300d\u306e\u5b9f\u73fe\u3092\u3081\u3056\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n<p style=\"padding-left: 30px\">\u2192<a href=\"http:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/topics\/\">\u300c\u500b\u5225\u30c6\u30fc\u30de\u300d\u3078\u623b\u308b<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>ATGC\u4ee5\u5916\u306e\u5929\u7136\u306b\u5b58\u5728\u3057\u306a\u3044\u300c\u6587\u5b57\u300d\u3092\u4f7f\u3063\u3066\u79c1\u305f\u3061\u304c\u958b\u767a\u3057\u305f\u6280\u8853\u306e\u3072\u3068\u3064\u306b\u3001\u300c\u7a81\u51fa\u672b\u7aef\u751f\u6210PCR\u6cd5\u300d\u304c\u3042\u308a\u307e\u3059\u3002\u82f1\u8a9e\u3067\u300cLACE (Ligation Assisted Cohesive Ending) -PCR\u6cd5\u300d\u3068\u540d\u2026 <span class=\"read-more\"><a href=\"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/topics\/alife\/\">\u7d9a\u304d\u3092\u8aad\u3080 &raquo;<\/a><\/span><\/p>\n","protected":false},"author":72,"featured_media":0,"parent":816,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/full-width.php","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-1555","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/pages\/1555","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/users\/72"}],"replies":[{"embeddable":true,"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/comments?post=1555"}],"version-history":[{"count":12,"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/pages\/1555\/revisions"}],"predecessor-version":[{"id":2314,"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/pages\/1555\/revisions\/2314"}],"up":[{"embeddable":true,"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/pages\/816"}],"wp:attachment":[{"href":"https:\/\/wps.itc.kansai-u.ac.jp\/mol-mach\/wp-json\/wp\/v2\/media?parent=1555"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}